A Novel High Resolution Human Metabolite MS/MS Spectral Library Enables Rapid and Accurate Metabolite Identification of Human Biofluids

Metabolite identification remains a major analytical challenge in metabolic profiling of human biofluids such as urine and serum/plasma. This article describes the creation of a high-resolution metabolite library of about 800 human metabolites established using a high performance Q-TOF spectrometer from Bruker. This library formed the basis for the described workflow for rapid and accurate metabolite identification in untargeted metabolomics research.