A Novel Solution for Vitamin K₁ and K₂ Analysis in Human Plasma by LC-MS/MS

Vitamin K₁ and K₂ analysis is typically complex and time-consuming because these lipophilic vitamins occur at low levels and are subject to matrix interference, particularly from phospholipids. The new method established here provides fast, accurate, and precise vitamin K analysis in plasma, using a simple phospholipid removal procedure and a sensitive, 4-minute LC-MS/MS analysis on a Raptor Biphenyl column, making it suitable for both research and high-throughput testing.