A Practical Approach to RT-qPCR - Publishing Data That Conform to the MIQE Guidelines

RT-qPCR is the method of choice for gene expression analysis because of its high sensitivity from samples of very low RNA concentrations. In an effort to assist the scientific community in producing consistent, high-quality data from qPCR experiments, the minimum information for publication of quantitative real-time PCR experiments (MIQE) guidelines have been published. This application note from Bio-Rad demonstrates how to apply the MIQE guidelines to establish a solid experimental approach.