A Quantitative Cell Invasion Assay Using ThinCert™ Cell Culture Inserts

This application note demonstrates a quantitative invasion assay performed with ThinCert™ cell culture inserts in a 24 well format. Thereby, the cells of interest are sowed onto the ECM coated PET membrane of the cell culture insert. Invasive cells are able to degrade the ECM and migrate to the underside of the membrane into the lower compartment that contains a chemoattractant. Subsequently the cells are fluorescently labelled, dislodged from the membrane and quantified in a fluorescence plate reader.