A Rapid, High Throughput Multiplex Assay that Identifies T-cell Subsets and Measures T-Cell Activation and Cytokine Secretion

T-cells play a critical role in the adaptive immune response. In naive T-cells, binding of the T-cell receptor (TCR) to peptides complexed with major histocompatibility complex (MHC) triggers an intricate signaling mechanism leading to T-cell activation, proliferation and production of cytokines. Modulating the TCR signaling pathway using biologics, small molecules or genetic engineering is highly relevant to many therapeutic areas including cancer immunotherapy, adoptive cell therapy, vaccine development and autoimmune diseases. The development of these drugs and therapies require the routine use of assays to profile T-cell function and health.