A Robust and Sensitive Capillary Flow LC – High-Resolution Accurate-Mass MS Platform for Discovery and Targeted Proteomics

This poster describes a reliable and easy-to-setup capLC-MS platform for sensitive targeted and shotgun analysis of peptides and proteins. The obtained results were compared with nano and analytical flow LC-MS analysis and recommendations for optimal LC and ESI interface configuration for different proteomics applications were formulated. The optimized capLC-MS conditions resulted in high-throughput separations with ~ 10 min total analysis time for targeted quantification of peptides at amole level.