A Robust Solid-State NMR Method for Protein Backbone Assignment: Band-Selective Homonuclear Cross Polarization

A crucial step for solid state NMR site-specific protein characterization and structure elucidation is the time-consuming resonance assignment. This application note presents a set of band-selective homonuclear cross polarization experiments to facilitate the recording of spectra and to simplify the assignment process. A detailed description of necessary parameters is given.