A Simple, Robust Automated Multiplex Assay for Mitochondria Toxicity Testing

Drug compound mitotoxicity, or toxicity within mitochondria, is now an important part of testing during drug discovery. Multiplexed cell-based mitochondrial assays increase sample throughput and decrease variability, costs and overall time for project completion. The utility of an automated multiplex assay to both assess and distinguish between cytotoxicity and mitochondrial function is presented in this application note from BioTek. The assay was carried out in both primary hepatocytes and immortalized HepG2 liver carcinoma cells. The combination of instrumentation, assay chemistry and primary hepatocytes is an ideal solution to accurately predict potential mitochondrial toxicity in lead compounds.