A Simplified Protocol to Streamline Inhibitor Profiling with the Transcreener® ADP2 FP Assay

Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes - many of which catalyze the covalent regulatory reactions that are central to cell signaling and are of great value as targets in drug discovery. The Transcreener® ADP² FP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of ADP and is a simple, one-step homogenous detection assay. This Application Note describes how to streamline the process of inhibitor profiling with the Transcreener® ADP² FP Assay eliminating the need to experimentally optimize the ADP² antibody concentration and the need for a standard curve.