A Universal LB-MSIA Workflow using Freedom EVO Platform for the Quantitative Analysis of Intact Therapeutic Antibodies of Different Allotype Subclasses

This application note demonstrates the effectiveness of quantitatively analyzing therapeutic mAbs of different constructs and allotype subclasses from rodent plasma using the Thermo Scientific™ enhanced capacity MSIA™ Streptavidin EVO microcolumns. This pre-clinical bioanalytical solution, on the Tecan Freedom EVO® platform, is based on mass spectrometric detection, specific for the bioanalysis of humanized, fully human and chimeric therapeutic monoclonal antibodies.