Accurately Count PBMC and Measure Viability in Presence of Residual RBC

One of the major issues of PBMC isolation is RBC contamination, which can be sampledependent. RBC contamination in a PBMC sample can introduce error to the measured concentration and viability, which can pass down to future experimental assays performed on these cells. To resolve this issue, RBC lysing protocol can be used to eliminate potential error caused by RBC contamination.