Achieving method modernization with the new liquid chromatographic gradient allowances provided by USP General Chapter <621> Chromatography and the Alliance iS HPLC System

The extent to which the various parameters of a chromatographic test may be adjusted without fundamentally modifying the pharmacopeial analytical procedures is defined in U.S. Pharmacopeia (USP) General Chapter <621> Chromatography. In this application note, Waters combines the gradient method adjustments described in this chapter with the Alliance™ iS HPLC System to achieve both column dimension and system modernization for the USP monograph separation of antiviral drug, abacavir sulfate.