Advanced HPLC Size-Exclusion Chromatography for the Analysis of Macromolecular Proteins Using 3.5 μm Ethylene Bridged Hybrid (BEH) Particles

This note will highlight the performance characteristics of both the 200Å and 450Å pore-size versions of these columns, designed for the separation of macromolecular proteins, with respect to resolution, column-to-column reproducibility, and column stability. Additionally, the distinct advantages in terms of resolution and sample-throughput of the sub-4-µm packing material over larger (5 and 8 µm) standard HPLC particle sizes for the separation of large proteins is shown.