Advances in CRISPR-Cas9 gene editing and screening technology

CRISPR-based gene editing enables researchers to make site-specific DNA edits resulting in permanent functional changes to the genome.

Originally discovered in bacteria as a defense mechanism against viral attack, the CRISPR-Cas system has since been adapted for a variety of genetic engineering applications. In this application eBook, we identify a number of applications for gene editing and functional genomics screening using Horizon Discovery’s Edit-R™ CRISPR genome engineering platform and cover:

• Gene knockouts
• Knock-in with homology-directed repair (HDR)
• DNA-free CRISPR-Cas9 gene editing
• Transfection optimization
• Editing stem cells and transgenic animals
• Functional genomic screening
• Transient activation or inhibition of endogenous genes (CRISPRa/CRISPRi)