Advancing Host Cell Protein Analyses Through the Combined Use of Microscale 2D RP/RP with CSH C₁₈ and Ion Mobility Enabled MS Detection

In this application note, recent advances in microscale peptide chromatography are applied to HCP analysis. The novel charge-surface-modified C₁₈ material, known as CSH C₁₈, which can produce high peptide peak capacities even at high mass loads, is used in the format of a 15K psi capable 300 μm I.D. column along with an ACQUITY UPLC M-Class based 2D-RP/RP system and SYNAPT HDMS^E detection to provide a robust analytical workflow for the identification of low concentrations of HCP impurities at single digit PPM levels (parts per million, or ng/mg).