Advantages of Online 2D Chromatography for MRM Quantification of Therapeutic Monoclonal Antibodies in Serum

In this application note, it is demonstrated that a two-dimensional chromatographic assay (2D LC or 2D UPLC) for multiple reaction monitoring (MRM) quantification in protein bioanalysis can provide up to a three-fold increase in sensitivity compared to one-dimensional LC/MRM. Additionally, high pH/low pH RP/RP separations can significantly reduce analyte suppression.