An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids

Disadvantages associated with 3D spheroid culture include the requirement for large numbers of cells; difficult manual processing steps; and a high overall cost per method. Recently developed 3D cell culture technologies, which have the ability to create spheroids from smaller cell numbers in high density microplates, can overcome the limitations of earlier methods while still providing the necessary environment for proper stem cell differentiation. This application note demonstrates the validation of a solution to perform automated chondrocyte differentiation from 3D hMSC spheroids.