An Integrated Platform for Real-Time Dynamic Culturing and Analysis of Hypoxia with Single Cell Resolution

Hypoxia, or inadequate oxygen supply in tissues, is a hallmark of primary tumors, arising due to impaired vascularization and increased respiratory demand of rapidly proliferating cancer cells. This application note presents a study of the hypoxic responses of various cancer cells using the CellASIC^® ONIX2 Microfluidic System and assay-optimized plates. These techniques enabled precise control over micro-environmental conditions, including gas and media content and provide results which gave an in depth insight into cancer progression.