An integrated workflow for the analysis of oligonucleotides and their impurities by Agilent high-resolution LC/(Q-)TOF mass spectrometry

Oligonucleotides have become fast-growing modalities in recent years. Along with the development of these candidates comes the increased need for robust analytical methods and easy-to-use data analysis workflows to characterize them. In this application note, two automated and integrated oligonucleotide data analysis approaches were developed for the characterization of a target oligonucleotide and its related impurities using high-resolution accurate mass (HRAM) MS data.