Analysing Testosterone in Human Serum by UHPLC using High Efficiency Kinetex® 1.7μm C18 Core-Shell Columns

Analysis of testosterone levels as an indication of hormonal imbalance is highlighted in this application note from Phenomenex. Testosterone was extracted from human serum by strong anion exchange polymeric SPE and analysed using a Kinetex C18, 30 x 2.1 mm, 1.7 μm column and positive polarity ESI LC/MS/MS system. This technology offers higher efficiencies than traditional columns, producing greater chromatographic resolution, sensitivity, and higher peak capacities.