Analysis and purification of oligonucleotides by anion exchange and ion-pairing reversed phase chromatography

In this whitepaper, YMC Europe provides useful information for all chromatographers who deal with the analysis and/or purification of oligonucleotides. Therapeutic oligonucleotides (TOs) are synthetic DNA or RNA oligomers designed to target specific genes or transcriptional pathways with the purpose of treating a wide range of diseases, including cancer, genetic disorders, and viral infections. The focus of this whitepaper is on anion exchange chromatography (AEX) and ion pair reversed phase chromatography (IP-RP). For both modes, the main aspects are the selection of a suitable stationary and mobile phase as well as the optimum temperature.