Analysis of Cyclic Antidepressant Drugs in Human Plasma by UHPLC-APCI Single Quadrupole Mass Spectrometry

Antidepressants are important drugs in the treatment of mental health problems. As such, they are characterized for absorption, distribution, metabolism and excretion (ADME), and monitored for abuse, toxicology and efficacy. This application note from PerkinElmer demonstrates a fast, simple, and highly-effective method to quantify cyclic antidepressants in human plasma by combining a simple and robust sample preparation method with UHPLC and atmospheric pressure chemical ionization mass spectrometry (APCI LC/MS) analysis. This procedure has the potential to improve sample analysis throughput in clinical research and pharmacokinetic studies.