Analysis of Dabsyl Amino Acids using HPLC

The pre-column derivatization using HPLC for amino acids analysis has prevailed as a simultaneous separation method of multiple components using a selective, high-sensitivity detector and a reversed phase column. Many results of amino acids analysis using dabsyl chloride as a reagent for pre-column derivatization have been reported because the amino acids derivatized by dabsyl chloride are more stable than those by other derivatization agents. Also a simply a UV/VIS detector can be used with high sensitivity. This application note demonstrates the measurement of the standard mixture of amino acids.