Analysis of hetero-oligomers with CG-MALS

Biomolecular interactions are commonly studied using binding assays. Quantitative binding assays hopefully provide the investigator with a unique value for binding affinity. Affinity is a key energetic parameter required to model how molecules A and B interact. Molecules A and B may bind with different stoichiometries at different molar ratios of A and B. Adding to this complexity, A and B may also self-associate into homo-oligomers.

Explore a method that disentangles all these possibilities in a single experimental run using multi-angle light scattering. Composition-gradient multi-angle light scattering (CG-MALS) provides easily interpreted data for complicated macromolecular interactions. Discover how a simple protocol provides information-rich data with a completion time of a few hours.