Analysis of Human IgG Glycans on a Solid Core Amide HILIC Stationary Phase

This application note demonstrates the analysis of human IgG glycans labeled with a fluorescent tag (2-aminobenzamide) by hydrophilic interaction liquid chromatography (HILIC). Glycans are oligosaccharides and polysaccharides found on proteins and cell surfaces. These entities play fundamental roles in cellular function by creating a fingerprint tag for the protein they are bound to, which in turn affects cellular activity. Glycans are often key biomarkers for disease states such as cancer. Due to the branching of the chains and post-translational modifications, their structures are very complex.