Analysis of Hydrophobic and Hydrophilic Silica TLC Plates

In this report, LAESI-MS is used to image molecules separated on a bare silica Thin Layer Chromatography (TLC) plate using the Protea LAESI® DP-1000 system and its proprietary ProteaPlot™ imaging software. This system can be used to analyze compounds separated on both hydrophilic and hydrophobic TLC silica plates, by simply wetting the TLC plates with water. This analytical method can be used to determine both the location and confirm the identity of analytes separated on TLC plates.