Analysis of Lamotrigine and its Glucuronide Metabolite in Water by Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry (LC/Q-TOF-MS) after Automated Solid Phase Extraction

This application note describes the conditions necessary to automate the solid phase extraction of lamotrigine and its 2-N-glucuronide metabolite from water samples prior to analysis by LC/Q-TOF-MS using the Gilson GX-271 ASPEC™ System. Extraction recoveries ranged from 75% to 99%. The RSD for inter-day (n=5) values were between 5% and 10%, which showed good reproducibility of the methodology. The LC/Q-TOF-MS limit of detection for lamotrigine and its metabolite were 1 ng/L and 5 ng/L.