Analysis of mRNA poly-A sequence variants by high-resolution LC-MS

The urgency engendered by the SARS-CoV-2 pandemic has prompted policymakers and pharmaceutical firms alike to develop and deploy mRNA vaccines with unprecedented speed. This application note demonstrates the use of an Agilent AdvanceBio 6545XT LC/Q-TOF to analyze poly-A tail sequences formed by E. coli poly-A polymerase (PAP), which is a common component of in vitro transcription systems.