Analysis of oligonucleotides with ion exchange chromatography and Agilent Infinity II Bio LC system

In this application note, Agilent Technologies demonstrates the effectiveness of using its Bio SAX columns for oligonucleotide analysis, which is better suited for certain analyses compared to ion-pair reversed-phase chromatography. The Bio SAX was able to conduct high-resolution ion exchange chromatographic separations of DNA and RNA oligonucleotides over a broad range of sizes without being affected by corrosion or oxidative damage from eluting salts. This application note demonstrates the versatility of ion exchange chromatography for oligonucleotide analysis and purification processes, and it provides a good starting point for further method optimization.