Analysis of phosphatidylethanol in whole blood using SPE and UPLC-MS/MS

Phosphatidylethanol (PEth) species are direct biomarkers of alcohol consumption that have attracted increasing interest recently due to their selectivity and relatively long half-life compared to other alcohol biomarkers. In this application note, discover the recent method developed by Waters for the extraction of PEth 16:0/18:1 and 16:0/18:2 from whole blood. Furthermore, this method features Ostro Pass-Through Sample Preparation plates, an ACQUITY UPLC® I-Class PLUS System and the Xevo™ TQ-S micro to achieve the performance and analytical sensitivity necessary for this method.