Analysis of Proteins by Size-Exclusion Chromatography Coupled with Mass Spectrometry Under Non-Denaturing Conditions

This application note describes SEC-MS under non-denaturing conditions. While this application has been evaluated for SE-HPLC, UPLC Technology in combination with sub-2-μm SEC column packing and a time-of-flight mass spectrometer (Xevo G2 Q-Tof) allows for direct analysis with improved resolution and sensitivity. By combining MS conditions with a Xevo G2 Q-Tof, SE-UPLC-MS analysis can be used to assist in the identification of unknown components in a biotherapeutic.