Analysis of Serum 17-Hydroxyprogesterone, Androstenedione, and Cortisol by UPLC-MS/MS for Clinical Research

Measurement of 17-hydroxyprogesterone (17-OHP) by immunoassay is prone to analytical interference arising from cross-reactivity of reagent antibodies with structurally-related steroid metabolites. In this application note, a method for the extraction and UPLC®-MS/MS analysis of serum 17-OHP and two additional adrenal steroids, androstenedione (A4) and cortisol, using the ACQUITY UPLC System with the Xevo TQ MS is described.