Analysis of Skeletal Remains Using Deep mtDNA Sequencing

Mitochondrial DNA (mtDNA) sequencing is useful in analyzing samples from mass graves due to its abundance, low mutation rate and maternal inheritance. The Illumina MiSeq system is a fully integrated NGS instrument, providing a rapid workflow and streamlined sample preparation that is ideal for sequencing mtDNA. This application note describes the use of the MiSeq system for deep sequencing of the HVI and HVII regions in mtDNA to aid in the identification of human skeletal remains in Vietnam.