Analysis of Small Drug Compounds on PRP-1 Polymeric Reversed Phase Columns

Reversed phase (RP) silica is still the most frequently used HPLC stationary phase despite some well-known limitations, such as pH and temperature stability, peak tailing or sensitivity for hydrolysis. Polymer-based resins on the contrary are not prone to these limitations and offer a good alternative for low and high pH applications. In this study the performance of Hamilton PRP-1 polystyrene-divinylbenzene RP-column has been compared to two C18 silica stationary phases for the separation of sibutramine, an antiobesity drug, using paroxetine as an internal standard.