Analysis of total RNA on the Agilent Femto Pulse System with the Agilent Ultra Sensitivity RNA kit

In this application note, Agilent explores the analysis of Total RNA using the Agilent Femto Pulse System and Agilent Ultra Sensitivity RNA Kit. The Femto Pulse system is a powerful pulsed-field capillary electrophoresis system designed for quantification, sizing, and quality control of nucleic acid samples. It promises superior sensitivity compared to gel electrophoresis, making it ideal for low-concentration samples and high molecular weight DNA. The Agilent Ultra Sensitivity RNA kit enables assessment of total RNA and mRNA with optimized methods for detection and quantification. The system provides reliable concentration, sizing, and quality metrics, such as the RNA quality number (RQN). The analysis demonstrates reproducibility, quantification consistency, and correlation between the Femto Pulse system and Agilent Fragment Analyzer.