Analysis of Vitamin D in Milk and Infant Formula using UHPLC/MS/MS per AOAC Method 2011.11

Fortification of milk, dairy products, infant and adult nutritionals with vitamin D is required in many countries in order to provide additional dietary vitamin D. A method for the determination of nutritionally important vitamin D forms is presented here. Analysis is performed as standards, and extracted from milk and infant formula after saponification following AOAC method 2011.11 using our Titan™ C18 UHPLC column and MS/MS with APCI detection.