Analyzing Bevacizumab, its aggregates and fragments

Due to the sizes of monoclonal antibodies (MAbs, about 150 kDa), size exclusion chromatography (SEC) is a standard technique for analysing MAbs such as Bevacizumab (Avastin^®). In this application note, YMC’s dedicated SEC column for antibodies, YMC-SEC MAB, is used to separate the monomer antibody from its aggregates as well as from degradation products in a single run.