Assay Assembly for Miniaturized Quantitative PCR in a 384-well Format Using the Echo® Liquid Handler

The capabilities of the Echo liquid handler to dispense into 384-well qPCR plates with total reaction volumes as low as 250 nL is highlighted in this application note. Standard deviations of less than 0.25 and CVs of less than 2% are seen routinely across plates using the low volume dispensing of the Echo liquid handler. The ability of the Echo liquid handler to transfer from any well to any well simplifies assay setup. The results demonstrate the capabilities to enable assay setup with flexible plate layouts, reduced reagent volumes and increased throughput.