Assay Development for Epigenetic Regulation of Histone 3 Lysine 36 Methylation Using AlphaLISA

In the last several years the field of histone methylation biology has grown significantly. However, the development of new cellular assays for methylating and demethylating enzymes has lagged behind, relying largely on reporter or high content assays. This application note demonstrates the development of a cell based AlphaLISA assay for the detection of dimethylation of histone H3 at lysine 36. The Maxcyte STX system enabled cDNA transfection efficiencies greater than 90% for cellular electroporation.