Assessing Autophagy with the guava easyCyte™ Benchtop Flow Cytometer

Autophagy is an intracellular catabolic pathway which causes cellular protein and organelle turnover, and is associated with diverse diseases such as Alzheimer’s disease, cancer, and Crohn’s disease, in addition to aging. It is a tightly regulated process that plays a normal part in cell growth, development, and cellular homeostasis. Download this application note to learn about optimized assay kits specifically designed for autophagy evaluation by flow cytometry and/or imaging. Unlike current flow cytometry assays for autophagy, these assays can discriminate between cytosolic and autophagosome associated LC3, thereby enabling robust, accurate evaluation of autophagy.