Assessing Gonadotropin Receptor Function by Resonance Energy Transfer-Based Assays

This white paper describes a study in which a series of novel BRET (Bioluminescence Resonance Energy Transfer) and FRET (Förster (Fluorescence) Resonance Energy Transfer) assays to study the activation and regulation of the human gonadotropin receptors. BRET and FRET have significantly contributed to major recent advances in the better understanding of GPCRs in physiology and pathophysiology. In the study, the correct expression of the different fusion proteins used for BRET and FRET was examined by fluorescence and luminescence measurements using a Mithras LB 943 plate reader (Berthold Technologies GmbH).