Assuring Optimal Promega Assays Development by Design of Experiment Workflow and Mantis Dispenser

To facilitate assay development a Design of Experiment approach can be employed during assay development. Design of Experiments (DoE) offers a systematic way of simultaneously evaluating multiple assay parameters and analyzing the resulting process outputs to optimize assay conditions with a minimum number of experiments. One of the limitations in taking full advantage of the benefits offered by DoE is the complexity of experimental set up. This poster demonstrates use of the Mantis instrument from Formulatrix, simplifying DoE setup by providing the unique capabilities of dispensing small volumes of multiple assay components with minimal dead volume into randomly designated wells. Flexibility in the dispensing capabilities and user friendly programming makes this instrument well suited for DoE workflow.