Automated 3D Cell Culture and Screening by Imaging and Flow Cytometry

Three-dimensional (3D) cell cultures offer greater physiological relevance than monolayer cultures for cellular interaction studies and compound screens. However, manual manipulations of these cultures can be laborious and challenges are amplified as sample throughput increases. This application note presents the use of the Biomek FXP Workstation in the automation of culture and drug sensitivity screening of cancer spheroids in Perfecta3D® Hanging Drop Plates. The transfer and dissociation of the spheroids into single cell suspensions was automated to enable flow cytometry analysis.