Automated Acquisition of MALDI-ISD Spectra for the N- and C-terminal Sequence Determination of Intact Proteins

For over 10 years, MALDI-Top-Down Sequencing (MALDI-TDS) of intact proteins has proved particularly useful for the determination of N- and C-terminal protein sequences. This technology note describes a procedure using the recently introduced 1,5-diaminonaphthalene (DAN) matrix and the new ultrafleXtreme™ MALDI-TOF/ TOF that automates the entire analysis - from sample preparation and data acquisition to N- and C-terminal sequence assignments.