Automated and efficient isolation of single cells into low volumes for scWGA

Tumors and tumor-derived cancer cell lines comprise a mix of diverse and heterogeneous cells. To interrogate the complex genetic makeup underlying tumorigenesis, cancer progression and metastasis, it is essential that researchers can analyze the genomes of individual cells. A prerequisite for these analyses is whole-genome amplification (WGA). Successful WGA from individual cells requires efficient isolation of single cells and their transfer into low-volume WGA reactions. iotaSciences demonstrates how GRIDs and the scPicking Platform supports the isolation of individual tumor cells for subsequent single-cell WGA (scWGA).