Automated Cell Synchrony Determination Using Microscopic Imaging in Live Cells

The effect of compounds on cellular proliferation is a key element of the ADME/Tox drug discovery process. For this reason the assessment of cell cycle progression under experimental conditions is highly desirable. This poster demonstrates that cells actively duplicating their DNA (S-phase) can be identified using incorporation of EdU into cellular DNA with subsequent labeling with fluorescent tags via Click-iT® chemistry and imaging with the Cytation™ 3 multimode reader.