Automated cloning for CRISPR workflows

Gene editing workflows require transformation of a large number of cells, followed by isolation of individual cells from the larger population to establish clonal colonies. Given the large number of gene edits required for contemporary research and the labor-intensive components of the workflow, there is an unmet need to automate post-transformation cloning. In this application note, Cell Microsystems presents data and protocols describing the CellRaft AIR® System as a fully automated cloning platform. Using an imaging-based sorting modality, single cells can be monitored for transformation-positive phenotypes as well as their retention of clonality as colonies begin to grow.