Automated DNA Library Construction Using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina® on the Beckman Coulter Biomek FXP Automated Liquid Handler

The ability to construct DNA sequencing (DNASeq) libraries from a wide range of starting input masses is essential for many next generation sequencing applications. This application note describes the automation of the New England Biolabs NEBNext Ultra DNA Library Preparation Kit for Illumina on the Beckman Coulter Biomek FXP Dual-Arm Multichannel 96 and Span-8 automated liquid handler (Biomek FXP). The automated method allows the user to prepare up to 96 individually indexed, high-quality, sequence-ready, DNASeq libraries in approximately 4-1/2 hours, from as little as 5ng of starting DNA.