Automated Extraction of Isomeric Cortisone and Prednisolone from Plasma using Novum™ Simplified Liquid Extraction (SLE) and Analysis by a Kinetex® Core-Shell Biphenyl HPLC/UHPLC Column

Corticosteroids exhibit anti-inflammatory properties and are widely abused within the sports industry. Chromatographic separation is essential for detection and confirmation of corticosteroids because cortisone and prednisolone are isomeric compounds. A simple automated extraction method using Phenomenex’s Novum Simplified Liquid Extraction (SLE) is employed to extract corticosteroids from plasma samples. A Kinetex 2.6 μm Biphenyl HPLC/UHPLC column is used to successfully separate these two compounds.