Automated, High Precision Tryptic Digestion and SISCAPA-MS Quantification of Human Plasma Proteins Using the Agilent Bravo Automated Liquid Handling

Quantitative reproducibility of tryptic digestion is essential to support the evolution of mass spectrometric assays for identification and quantitation of peptides, and ultimate measurement of proteins as clinically relevant biomarkers.
This Application Note presents the development of a highly reproducible, automated “addition only” method for tryptic digestion of plasma, followed by the previously-described bead-based SISCAPA enrichment3 of target peptides and quantitation using TQMS.